chromatography, Stationary Phase, Mobile Phase, and type of chromatography

 CHROMATOGRAPHY:

     chromatography is an important biophysical technique that enable the separation, identification & purification of the components of a mixture for qualitative and quantitative analysis.

STATIONARY PHASE:

     The stationary phase in chromatography is the phase that is either a solid & liquid particle attached to a glass or a metal surface on which the components of the mixture to be separated is absorbed selectrively.

MOBILE PHASE:

     The mobile phase is chromatography is the phase that either liquid (or) gas that passed through a chromatography system where the components of the mixture are separated at different rates by adsorbing them to the stationary phase.

TYPE OF CHROMATOGRAPHY:

• Affinity
• Anion
• Cation
• Column
• Flash
• Gas
• Gel filteration
• High-Performance liquid (HPLC)
• Hydrophobic Interation
• Ion Exchange
• Liquid
• Paper
• Reverse-Phase
• Thin-Layer (TLC)